Human/MonKey/Mouse E2 ELISA Kit from Innovative Research is a highly sensitive competitive enzyme-linked immunoassay (ELISA) for measuring E2 in serum, plasma, urine, and saliva. Reagents for up to 96 tests.This ELISA Kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this Kit has been pre-coated with Human/MonKey/Mouse E2. During the reaction, Human/MonKey/Mouse E2 in samples or Standard competes with a fixed amount of Human/MonKey/Mouse E2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human/MonKey/Mouse E2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ▒ 2 nm. The concentration of Human/MonKey/Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.More Details: Detection Target: E2 Reactivity: Human/MonKey/Mouse Range: 9.37 - 600pg/mL Sensitivity: 5.63pg/mL Storage Conditions: An unopened kit can be stored at 2 - 8 ░C for 1 month unless otherwise specified in the kit documetation. If the kit is not supposed to be used within 1 month, store the components separately upon receipt according to the directions accompanying the kit., At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.For research use only.