Mouse Glucose Transporter 1 ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Glucose Transporter 1 in biofluid samples, such as tissue homogenates, cell lysates or other biological fluids.This is a sandwich ELISA in strip well format with reagents for up to 96 tests. GLUT1 present in samples reacts with an anti-Mouse GLUT1 capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-GLUT1 detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active GLUT1 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse GLUT1. Detection Target: Mouse Glucose Transporter 1 Reactivity: Mouse Range: 1.25-80ng/mL Sensitivity: 0.56ng/mL Storage Conditions: Store all components at 4┬░C This Mouse GLUT1 activity assay is for the quantitative determination of Glucose Transporter 1 in samples including tissue homogenates, cell lysates or other biological fluid For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.